PhD in Neuroscience

This project, part of the LIGHTinPARIS program co-funded by University Paris Saclay and the Marie Skłodowska-Curie Actions Program within the European Commission MSCA framework, investigates how central brain circuits and motor circuits interact to shape learning, memory, and action inDrosophilalarvae./nUsing dual-color imaging, SLM and closed-loop photo-stimulation, we will simultaneously record and manipulate identified neurons in the memory center (mushroom body) and in motor pathways with high spatial and temporal precision./nProject ObjectivesOur goal is to use advanced optics to characterize interactions between the learning and memory center and the motor system inDrosophilalarvae, and to deliver a functional dataset for computational modeling. Aim 1: Determine synchronicity between memory and motor neurons. We will (1.1) monitor calcium dynamics in neurons labeled with distinct fluorescent sensors, (1.2) manipulate neuro-modulation pharmacologically, and (1.3) develop analysis tools to quantify synchronization between brain and motor activity. Aim 2: Test ascending motor-to-central brain influences. We will photoactivate motoneurons with spatial light modulation to mimic motor programs (2.1), and assess how these signals are encoded in the memory center (2.2). Aim 3: Test descending brain-to-motor effects. We will activate memory neurons while monitoring fictive locomotion (3.1), and use closed-loop optogenetics on a Karthala two-photon microscope to determine how descending signals modulate ongoing turns (3.2).